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des [2018-08-12 19:13:34]
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des [2018-08-12 19:13:34]

phospholipid desaturase
locus
BSU19180
pI
9.88
mw
40.55 kDa
protein length
352 aa Sequence Blast
gene length
1056 bp Sequence Blast
function
adaptation of membrane fluidity at low temperatures
product
phospholipid desaturase
essential
no
synonyms
yocE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,089,396 → 2,090,454

    Phenotypes of a mutant

  • cold-sensitive [Pubmed|19820084]

    • The protein

    Catalyzed reaction/ biological activity

  • introduces double bonds into long chain fatty acids [Pubmed|21665975]

    • [SW|Cofactors]

  • [protein|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|Des] receives electrons from ferredoxin ([protein|4164F07C581320D67FDC1ED5014272B81702A371|Fer]) or from either of the two flavodoxins, [protein|0932DDC285635C02067A3302CEDA302FE4269179|YkuN] or [protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|YkuP], the three proteins can functionally replace each other [Pubmed|21665975]

    • [SW|Localization]

  • integral membrane protein [Pubmed|21665975]

    • Expression and Regulation

    Operons

    genes
    [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]
    description
    [Pubmed|10559169]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10559169], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR]: activation, [Pubmed|11285232], [Pubmed|19820084], in [regulon|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR regulon]

    • regulation

  • induced upon cold shock ([protein|search|DesR]) [Pubmed|11285232]

    • additional information

  • in strains evolved for growth at low pressure, 'des' expression is significantly increased as compared to the wild type [PubMed|22416764]
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    Biological materials

    Mutant

  • MGNA-A319 (yocE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/319 NBRP B. subtilis, Japan]
  • 1A835 ( ''des''::''kan''), [Pubmed|11285232], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A835&Search=1A835 BGSC]
  • BKE19180 (Δ[gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTAAGTATCCTCTCAT, downstream forward: _UP4_TGATGATAAAGGAGGTCTTC
  • BKK19180 (Δ[gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTAAGTATCCTCTCAT, downstream forward: _UP4_TGATGATAAAGGAGGTCTTC

    • Labs working on this gene/protein

  • [SW|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]

    • References

    Reviews

  • 19363032

    • Original publications

  • 19164152,11251847,12399512,9555904,10559169,11285232,12207704,12730185,19820084,12207704,20581210,22416764,18154726,21665975,26647299,28461449